Serial Cloner can be downloaded as freeware for Mac and Windows OS and is definitely worth having if you carry out PCR and cloning on a regular basis. Of course, if you are amplifying your target from a plasmid, the risk of unspecific PCR products will be lower. National Centre for Biotechnology Information (NCBI)ĭid you know that the NCBI website can be used for so much more than literature and BLAST searches? At primer blast you can search for suitable primer pairs (much like Primer3), or you can enter specific primer sequences and check their specificity by performing a primer blast, helping you to avoid off-target amplification. BLASTing your primers against your genome of interest might be particularly useful if you are trying to amplify a gene that exists as part of a multigene family if your template is total genomic DNA. ![]() You can also use Primer3 to design hybridization probes for use in PCR-ELISA and other probe-based applications.Ģ. You can choose to refine your results in several ways, including the size of your desired PCR product, primer size, Tm range, and other parameters. ![]() You simply paste in your target sequence and search for primers. Primer3 is a very simple yet efficient online interface for primer design. Looking for new PCR reagents and equipment.Estimating PCR efficiency from the slope of an amplification curve so you don’t need to run standard curves in qPCR.Relative gene expression analysis for your raw qPCR data.Restriction mapping of your PCR product to aid cloning.Calculating general properties of primers and amplicons (Tm, secondary structure, primer dimers).How do you sort out the > 20 million hits you get when you Google ‘PCR’? This list of the Top 10 PCR websites contains those used by myself and colleagues and should be a good go-to for any of the following PCR issues: Many of the program's tools will only be familiar to scientists and academics but there's also a web browser for the direct import of NCBI and EMBL entries.Whether you are embarking on a PhD in molecular biology, trying to amplify a new gene, analyzing gene expression by qPCR, or trying to solve a PCR problem, you will probably resort to a Google search at some point or another. Obviously, the main aim of the program is to provide an easy way to clone DNA - a task which it does very well and mainly just using a graphical interface. You can numerically select fragments and carry out calculations of peptides which I have no idea about but certainly look impressive. All the tools you need to analyze and manipulate your DNA sequences are available in a very simple and easy to read all-in-one-window. The program includes a whole host of mapping options such as genetic maps, fragment viewers and a virtual PCR. It is therefore an extremely powerful piece of software that will only be of interests to scientists and science-biology students. Serial Cloner is an application that allows you to read and write files DNA sequences that conform to the FASTA and pDRAW32 format. ![]() Until now, it reading and writing it was a very specialised field but this program intends to bring it to the masses. DNA is the complex code that makes up the lifeform in all of us.
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